GBSX

Restriction Enzyme Predictor

mandatory parameters:
* -d digest sequence
* -l read length
* -f file of reference fasta file location(s)

optional parameters:
* -e enzyme name to use (default: Enzyme)
* -g genome name to use in bed file name (default: genome)
* -n minimum size fragments to include (default: 100)
* -m maximum size fragments to use (default: 1000)
* -E second enzyme name to use (default: Enzyme2)
* -D digest sequence for a second enzyme (default: not declared)
* -R digest sequence for a third enzyme (default: not declared)